Usefulness of rifabutin loaded floating beads for the treatment of Helicobacter pylori infection in experimental Mongolian gerbils
Keywords:
Rifabutin, floating beads, gellan gum, Helicobacter pyloriAbstract
The objective of the present study was to develop stomachspecific drug delivery system of rifabutin and to evaluate their effectiveness in eradication of Helicobacter pylori in experimental Mongolian gerbil model. Rifabutin loaded floating gellan gum beads prepared by calcium induced ionotropic gelation in acidic medium. The prepared beads were evaluated for in vitro characterization and in vivo Helicobacter pylori clearance efficiency following repeated oral administration to Helicobacter pylori infected Mongolian gerbils. Our results showed that the rifabutin loaded floating beads possess significant anti- Helicobacter pylori effect in the in vivo gerbil model. The findings support that rifabutin floating beads could be a promising gastrointestinal drug delivery system in treatment of Helicobacter pylori.
References
Vandenplas Y. Helicobacter pylori infection.
World J Gastroenterol. 2000;6:20-31.
Yuichi S, Mitsuya I, Jun-Ichi U, Akira Y,
Tsutomu M, Hirotaka M, Kazuhito S, Terasu
H, Rintaro N, Takafumi I, Hitoshi A, David
H. Gastric Carcinoid Tumors Without
Autoimmune Gastritis in Japan: A
Relationship with Helicobacter pylori
Infection. Dig Dis Sci. 2002;47:579-585.
Ricci VC, Ciacci C, Zarrilli R, Sommi P,
Tummuru MK, Del Vecchio Blanco C,
Bruni CB, Cover TL, Blaser MJ, Romano
M. Effect of Helicobacter pylori on gastric
epithelial cell migration and proliferation in
vitro: role of VacA and CagA. Infect
Immun1996;64:2829–2833.
Heep M, Beck D, Bayerdorffer E, Lehn N.
Rifampin and Rifabutin Resistance
Mechanism in Helicobacter pylori.
Antimicrob Agents Chemother.
;43:1497-1499.
Kunin CM. Antimicrobial activity of
rifabutin. Clin Infect Dis. 1996;c22(Suppl
:S3– 13.
Alberto P, Marilisa F, Mario R, Francesca F,
Mariuccia S. In vitro activity of rifabutin
against strains of Helicobacter pylori
resistant to Metronidazole and
Clarithromycin. Am J Gastroenterol.
;95:833-834.
Rajnikanth PS, Mishra B. Preparation and in
vitro characterization of gellan based
floating beads of acetohydroxamic acid for
eradication of H. pylori. Acta Pharm.
;57:413–427.
Choi BY, Park HJ, Hwang SJ, Park JB. Prep
aration of alginate beads for floating drug
delivery: effects of CO2 gas forming
agents. Int J Pharm. 2002;239:81-91.
Benoit B, Lina B, Miche`le P, Jose´e C,
Raymond D. LIVE/DEAD BacLightE:
application of a new rapid staining method
for direct enumeration of viable and total
bacteria in drinking water. J Microbiol
Methods. 1999;37:77–86.
United State Pharmacopeia 31/National
Formulary 26, Vol 3. USP Convention,
Rockville, MD 20852. Asian Edition,
;pp3181-3182.
Stockwell AF, Davis SS. In vitro evaluation
of alginate gel system as sustained release
drug delivery systems, J Control
Release.1986;3:167–175.
Buzas GM, Szekely E, Illyes G, Szeles I.
Lansoprazole versus ranitidine bismuth
citrate containing triple regimens for
Helicobacter pylori eradication in patients
with duodenal ulcer. Orv Hetil.
;141:1711-1714.
Warren J R, Marshall B. J. Unidentified
curved bacilli on gastric epithelium in active
chronic gastritis. Lancet. 1983;I:1273–1275.
Catalano F, Branciforte G, Catanzaro R,
Bentivegna C, Cipolla R. Nuciforo G,
Brogna A. Comparative treatment of
Helicobacter pylori-positive duodenal ulcer
using pantoprazole at low and high doses
versus omeprazole in triple therapy.
Helicobacter. 1999;4:178-184.
Velasco Benitez CA, Amorocho Perez RD.
Treatment of gastritis-associated
Helicobacter pylori infection in children.
Rev Gastroenterol Peru 2002;22:159-163.
