Effect of Penetration Enhancer DMSO on In-Vitro Skin Permeation of Acyclovir Transdermal Microemulsion Formulation
Keywords:
DMSO, Penetration enhancer, Ethanol, Transdermal Microemulsion, Acyclovir (ACV)Abstract
The aim of this research was to enhance the flux of transdermal drug delivery by using penetration enhancers DMSO. Skin penetration enhancers have been used to improve bioavailability and increase the range of drugs for which topical and transdermal delivery is a viable option which penetrate into skin to reversibly decrease the barrier resistance. Penetration enhancing activity of dimethylsulphoxide (DMSO) at 5% w/w and 10% w/w concentration were determined in aqueous solution of ACV and in microemulsion formulations though calculation of transdermal flux of ACV with Keshary Chein Frenz Diffusion cell by using wistar albino rat skin. The transdermal flux of formulations PD, PD5D, PD10D, ME1 and ME10D was found to be 2.47, 50.7529, 119.7691, 238.1432 and 266.6721µg/cm2/h. The flux of microemulsion formulation ME10D was found 266.6721± 8.49 µg/cm2/h. Which showed highest value and skin flux of the drug could be enhanced up to 107 fold compared to its aqueous solution by preparing microemulsion ME10D. DMSO in microemulsion formulation is safe to the skin at 10% DMSO w/w. Keywords: DMSO, Penetration enhancer, Ethanol, Transdermal Microemulsion, Acyclovir (ACV)
References
AC, Barry BW. Penetration
, Iglesias-Vilas G, BlancoThe significant difference in acyclovir
permeation between microemulsion formulations,
PD and ME10D was probably due to the mean
size of internal phase droplets, which were
significantly smaller in microemulsions and
effect of DMSO penetration enhancer.
Conlusion
We have show
penetration enhancer for the transdermal delivery
of ACV and skin flux of the drug could be
enhanced up to 107 fold compared to its aqueous
solution. Increased percentage of DMSO 10% as
compared to 5% in aqueous solution enhanced
transdermal flux 2.36 fold greater. The skin flux
of the drug is dependent on the concentration of
water, DMSO and ethanol in microemulsion.
Microemulsion is safe to the skin at 10% DMSO
w/w.
Ackn
Authors are great thankfu
Pharmacy, Bundelkhand university Jhansi, (UP)
India, for providing guidance, laboratories and
animal facilities. Authors are great thankful to
Cipla Ltd, Mumbai, for providing Acyclovir as
free gift sample. Authors are also thankful to
NICAIR Delhi and AIIMS New Delhi for
literature survey and TEM facilities.
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