Dietary Lutein Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
Keywords:
Branchoalveloar lavage fluid, C57BL/6J mice, hydroxyproline, lutein, inflammation, oxidative stress, superoxide dismutaseAbstract
Idiopathic Pulmonary Fibrosis is a chronic lung disease characterized by lung inflammation and progression to fibrosis. Anti-fibrotic efficacy of lutein was studied in mouse model of bleomycin induced pulmonary fibrosis. The mice were orally administrated with 100 or 200 mg/kg of lutein three days prior to intra-tracheal instillation of bleomycin on day 0 and continued up to day 21. Total cell counts and protein levels in branchoalveolar lavage fluid and superoxide dismutase, and hydroxyproline levels were evaluated. Treatment with lutein at 100 and 200 mg/kg prevented bleomycin induced mortality and body weight loss. Lutein administration attenuated bleomycin induced increase of total and differential cell count and myeloperoxidase, inflammatory cell infiltration in lung tissue. Similarly lutein administration restored superoxide dismutase activity lowered by bleomycin instillation. The combined results revealed the protective effect of lutein by suppressing the inflammation, oxidative stress and enhancing the antioxidant potential which may helps in pulmonary fibrosis.
References
King TE. Idiopathic pulmonary fibrosis. In: Schwarz MI, King TE, eds. Interstitial lung disease. St. Louis, MO: Mosby Year Book Inc; 1993, p. 367-403.
Mapel DW, Hunt WC, Utton R, Baumgartner KB, Samet JM, Coultas DB. Idiopathic pulmonary fibrosis: survival in Population based and hospital-based cohorts. Thorax. 1998; 53(6): 469-476.
Crystal RG, Bitterman PB, Rennard SI, Hance AJ, Keogh BA. Interstitial lungdiseases of unknown cause. Disorders characterized by chronic inflammation of the lower respiratory tract. N Engl J Med. 1984; 310(3): 154-66.
Coultas DB, Zumwalt RE, Black WC, Sobonya RE. The epidemiology of interstitial lung diseases. Am J Respir Crit Care Med. 1994; 150(4):967-72.
Thannickal VJ, Toews GB, White ES, Lynch JP, Martinez FJ. Mechanisms of pulmonary fibrosis. Annu Rev Med. 2004; 55: 395-417.
Tanaka K, Ishihara T, Azuma A, Kudoh S, Ebina M, Nukiwa T, Sugiyama Y, Tasaka Y, Namba T, Ishihara T, Sato K, Mizushima Y, Mizushima T. Therapeutic effect of lecithinized superoxide dismutase on bleomycin-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2010;298(3):348-360.
Sheppard D. Transforming growth factor beta: a central modulator of pulmonary and airway inflammation and fibrosis. Proc Am Thorac Soc. 2006; 3(5): 413-417.
Kinnula VL, Myllarniemi M. Oxidant-antioxidant imbalance as a potential contributor to the progression of human pulmonary fibrosis. Antioxid Redox Signal. 2008; 10)4):727-738.
Willis BC, Borok Z. TGF β1 induced EMT: mechanisms and implications for fibrotic lung disease. Am J Physiol Lung Cell Mol Physiol. 2007; 293(3): 525-534.
Khalil N, O Connor R. Idiopathic pulmonary fibrosis: current understanding of the pathogenesis and the status of treatment. CMAJ. 2004;171(2):153-160.
Chua F, J Gauldie, Laurent GJ. Pulmonary fibrosis: searching for model answers. Am J Respir Cell Mol Biol. 2005; 33(1): 9-13.
Phan SH, Thrall RS, Ward PS. Bleomycin-induced pulmonary fibrosis in rats:biochemical demonstration of increased rate of collagen synthesis. Am Rev Respir. 1995; 121(3): 501-506.
Luppi F, Cerri S, Beghe B, Fabbri LM, Richeldi L. Corticosteroid and immuno modulatory agents in idiopathic pulmonary fibrosis. Respir Med. 2004; 98 (11):1035-1044.
Sasaki M, Ozawa Y, Kurihara T, Noda K, Imamura Y, Kobayashi S, Ishida S, Tsubota K. Neuroprotective effect of an antioxidant, lutein,during retinal inflammation. Invest Ophthalmol Vis Sci. 2009; 50 (3):1433-1439.
Kim JE, Leite JO, DeOgburn R, Smyth JA, Clark RM, Fernandez ML. A lutein-enriched diet prevents cholesterol accumulation and decreases oxidized LDL and inflammatory cytokines in the aorta of guinea pigs. J Nutr. 2011;141 (8):1458-1463.
Jin XH, Ohgami K, Shiratori K, Suzuki Y, Hirano T, Koyama Y, Yoshida K, Ilieva I, Iseki K, Ohno S. Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats. Invest Ophthalmol Vis Sci. 2006; 47: 2562-2568.
Li SY, Fu ZJ, Ma H, Jang WC, So KF, Wong D, Lo AC. Effect of lutein on retinal neurons and oxidative stress in a model of acute retinal ischemia/reperfusion. Invest Ophthalmol Vis Sci. 2009; 50(2):836-43.
Krinsky NI, Landrum JT, Bone RA. Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Annu Rev Nutr. 2003; 23:171-201.
He RR, Tsoi B, Lan F, Yao N, Yao XS, Kurihara H. Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice. Chinese Medicine 2011; 6(1):38.
Furonaka M, Hattori N, Tanimoto T, Senoo T, Ishikawa N, Fujitaka K, Haruta Y, Yokoyama A, Kohno N. Suplatast tosilate prevents bleomycin-induced pulmonary fibrosis in mice. J Pharmacol Exp Ther. 2009;328:55-61.
Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale. J Clin Pathol. 1988; 41(4):467-70.
Yan XT, Tumpey TM, Kunkel SL, Oakes JE, Lausch RN. Role of MIP-2 in neutrophil migration and tissue injury in the herpes simplex virus-1- infected cornea. Invest Ophthalmol Vis Sci. 1998; 39(10):1854-1862.
Manikonda PK, Jagota A. Melatonin administration differentially affects age-induced alterations in daily rhythms of lipid peroxidation and antioxidant enzymes in male rat liver. Biogerontology. 2012;13:511-524.
Molina-Molina M, Pereda J, Xaubet A. Experimental models for the study of pulmonary fibrosis: current usefulness and future promise. Arch. Bronconeumol. 2007; 43(9): 501-507.
Raghu G, Brown KK, Bradford WZ, Starko K, Noble PW, Schwartz DA, King TE Jr. Idiopathic Pulmonary Fibrosis Study Group. A placebo-controlled trial of interferon gamma-1b in patients with idiopathic pulmonary fibrosis. N Engl J Med. 2004; 350:125-133.
Zhao L, Wang X, Chang Q, Xu J, Huang Y, Guo Q, Zhang S, Wang W, Chen X, Wang J. Neferine, a bisbenzylisoquinline alkaloid attenuates bleomycin-induced pulmonary fibrosis. Eur J Pharmacol. 2010; 627:304-312.
Tsai KD, Yang SM, Lee JC, Wong HY, Shih CM, Lin TH, Tseng MJ, Chen W. Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice. Evid Based Complement Alternat Med. 2011; 402761.
Ali EN, Mansour SZ. Boswellic acids extract attenuates pulmonary fibrosis induced by bleomycin and oxidative stress from gamma irradiation in rats. Chinese Medicine. 2011; 6:36.
Boyaci H, Maral H, Turan G, Basyigit I, Dillioglugil MO, Yildiz F, Tugay M, Pala A, Ercin C. Effects of erdosteine on bleomycin-induced lung fibrosis in rats. Mol Cell Biochem. 2006; 281(1-7): 129-137.
