Biosynthesis, characterization and antibacterial activity of silver nanoparticles by soil fungi Pencillium sps

Authors

  • G Swathi Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University,Tirupati- 517502Andhra Pradesh India
  • A Sridevi Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University,Tirupati- 517502Andhra Pradesh India
  • A Sandya Department of Microbiology and Biotechnology Sri Padmavati Mahila Visvavidyalayam,Tirupati-517502 India
  • B Praveen Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University,Tirupati- 517502Andhra Pradesh India
  • G Narasimha Applied Microbiology Laboratory, Department of Virology, Sri Venkateswara University,Tirupati- 517502Andhra Pradesh India

Keywords:

Silver nanoparticles, Biological synthesis, Characterization, Antibacterial activity

Abstract

In the present days microbial synthesis of nanoparticles is an eco-friendly green chemistry approach that correlates with nanotechnology and microbial biotechnology. In this study exposure of fungal biomass to aqueous 1Mm AgNO3 solution resulted in the reduction of the metal ions by the nitrate reductase enzyme present in the fungal cell wall membranes and formation of silver nanoparticles. Synthesized silver nanoparticles were characterized using UV-visible spectroscopy, SEM, TEM and FTIR analysis and size and shapes were determined. The synthesed silver nanoparticles were exhibited an excellent antibacterial activities against both gram negative and gram positive pathogenic bacterial strains which causes the diseases in human beings.

References

. Simkiss K, Wilbur KM. Cell Biology

and Mineral Deposition,

Biomineralization, Academic Press,

New York, 1989.

. Mann S (ed.). Biomimetic Materials

Chemistry, VCH Publishers, 1996.

. Southam G, Beveridge TJ. Geochim,

Cosmochim. Acta. 1996;60, 4369ă

. Beveridge TJ, Murray RGE. Sites of

metal deposition in the cell wall of

Bacillus subtilis. J. Bacteriol. 1980;

, 876ă 887.

. Li P, Li J, Wu C , Wu Q , Li J.

Synergistic antibacterial effects of blactam antibiotic combined with silver

nanoparticles. Nanotechnology.

;16, 1912ă1917.

. Panacek A, Kvitek L, Prucek R, Kolar

M, Vecerova R, Pizurova N, Sharma

VK, Tatjana N, Zboril Z. Silver colloid

nanoparticles: synthesis,

characterization, and their

antibacterial activity. J. Phys Chem. B,

;110,16248ă16243.

. Mohanpuria P, Rana KN, Yadav SK.

Biosynthesis of

nanoparticles:technological concepts

and future applications. J. Nanopart.

Res. 2008;10, 507- 517.

. Gericke M, Pinches A. Microbial

production of gold nanoparticles. Gold

bull. 2006;39(1): 22-28.

. S Harley. Use of a simple colorimetric

assay to demonstrate conditions for

induction of nitrate reductase in

plants. Am. Biol. Teacher. 1993;55:

. Sastry M, Mayya KS, Patil V,

Paranjape DV, Hegde SG. Langmuirblodgett films of carboxylic acid

derivatized silver colloidal particles:

role of subphase pH ondegree of

cluster incorporation. J. PhyChem B,

;101:4954ă4958.

. Kerker MJ. Colloid Interf. Sci. 1985

;105: 297.

. Sosa IO, Noguez C, Barrera RG.

Optical properties of metal

nanoparticles with arbitrary shapes. J.

Phys. Chem. B, 2003;107: 6269ă

. Brause R, Moeltgen H, Kleinermanns

K. Characterization of laser-ablated

and chemically reduced silver colloids

in aqueous solution by UV/VIS

spectroscopy and STM/SEM

microscopy, Appl. Phys. B, Lasers

Opt. 2002;75 -711.

. Mukherjee P, Ahmad A, Mandal D,

Senapati S, Sainkar SR, Khan MI,

Parischa R, Ajaykumar PV, Alam M,

Kumar R, M Sastry,Bioreduction of

AuCl4- ions by the fungus, Verticillium

sp. And surface trapping of the gold

nanoparticles formed. NanoLett.

;1: 515ă519

. Ahmad A, Mukherjee P, Senapati S,

Mandal D, Khan MI, Kumar R, Sastry

M. Extracellular biosynthesis of silver

nanoparticles using the fungus

FusariumoxysporumBiointerfaces.

; 28: 313ă318.

. Huang J, Chen C, He N, Hong J, Lu

Y, Qingbiao L, Shao W, Sun D, Wang

XH, Wang Y, Yiang X. Biosynthesis of

silver and gold nanoparticles by novel

sundried Cinna- momumcamphora

leaf. Nanotechnology. 2007;18 -105.

. Luo L, Yu S, Qian S, Zhou T. J. Am

Chem Soc. 2005;127 -2822.

. Caruso F, Furlong DN, Ariga K,

Ichinose I, Kunitake T. Langmuir.

; 14 -4559.

. Van de Weert M, Haris PI, Hennink

WE, Crommelin DJA. Fourier

transform infrared spectrometric

analysis of protein con- formation:

effect of sampling method and stress

factors. Anal Biochem. 2001;297 -160.

. [20] Morones JR, Elechiguerra JL,

Camacho A, Holt K, Kouri JB,

Ramirez JT, Yacaman MJ. The

bactericidal effect of silver

nanoparticles, Nanotechnology, 2005.

; 2346ă2353.

. MacKeen PC, Person S, Warner SC,

Snipes W, Stevens SE. Silver-Coated

Nylon Fiber as an Antibacterial Agent,

Antimicrob. Agents Chemother. 1987;

, 93ă99.

. Li Z, Lee D, Sheng X, Cohen RE,

Rubner MF. Two-Level Antibacterial

Coating with Both Release-Killing and

Contact-Killing Capabilities, Langmuir.

;22, 9820ă9823.

. Jeong SH, Hwnag YH, Yi SC. Ant b

cte l properties of padded PP/PE

nonwovens incorporating nano-sized

silver colloidsJ. Mater Sci. 2005;40,

ă5418.

. Lok CM, Ho CM, Chen R, He QY, Yu

WY, Sun H, Tam PK, Chiu JF, Che

CM. Proteomic analysis of the mode

of antibacterial action of silver

nanoparticles J. Proteome Res. 2006;

, 916ă924.

. Lee D, Cohen RE, Rubner MF.

Heterostructured Magnetic

Nanotubes, Langmuir. 2005;21,

ă9659.

. Thiel J, Pakstis L, Buzby S, Raffi M,

Ni C, Pochan DJ, Shah SI.

Antibacterial Properties of SilverDopedTitania, Small. 2007;3, 799ă

. Lee HJ, Yeo SY, Jeong SH.

Antibacterial effect of nanosized silver

colloidal solution on textile fabrics J.

Mater. Sci. 2003;38(10), 2199.

. Kokkoris M, Trapalis CC, Kossionides

S, Vlastou R, Nsouli B, Grotzschel R,

Spartalis S, Kordas G, Paradellis T.

RBS and HIRBS studies of

nanostructured AgSiO2 solăgel thin

coatings. Nucl.Instrum.Meth. B,

;188, 67.

Downloads

Published

2014-06-30

How to Cite

G Swathi, A Sridevi, A Sandya, B Praveen, & G Narasimha. (2014). Biosynthesis, characterization and antibacterial activity of silver nanoparticles by soil fungi Pencillium sps. International Journal of Drug Delivery, 6(2), 165–171. Retrieved from https://ijdd.arjournals.org/index.php/ijdd/article/view/242

Issue

Section

Original Research Articles