Preparation and Evaluation of Curcumin Invasomes
Keywords:
Curcumin, Cyclodextrin, Invasomes, HPMCK4M gelAbstract
Curcumin has poor aqueous solubility and has bioavailability problems. Hence in the present study the solubility of curcumin was increased by complexing with cyclodextrin (CD) and Hydroxy propyl β cyclodextrin(HPβCD). This complex is incorporated in to invasomes and then into HPMC gel to prepare as a transdermal formulation.Curcumin cyclodextrin complexes were prepared by physical mixture and co-precipitation method. Different formulations of invasomes containing 0.5, 1.0, 1.5 % of limonene, fenchone, nerolidol individually were prepared using mechanical dispersion technique. Invasomes were characterized for vesicular size, surface morphology, zeta potential, entrapment efficiency and percutaneous permeation.Formulations CHL1 and CHL2 were optimized for further studies. It was found in the study that complex with HPβCD in 1:2 proportion prepared by coprecipitation method was found to bind 90% of curcumin.Invasomal preparation with 0.5% limonene, 4% ethanol was found to enhance permeation by 8.11 times the control.In vivo diffusion studies were conducted using franz diffusion cell, ex vivo skin permeation studies of CHL1using rat abdominal skin showed cumulative drug permeated (Q24)of 70.32 øg/cm2, steady state transdermal flux of 3.344 øg/cm2/hr-1, permeability coefficient of 5.35 cm/hr and lag time of 1 hr when compared with control formulation. From the results it was concluded that the solubility of curcumin increased by complexing with HPβCD and invasomal preparation with 0.5% limonene has improved permeation through the skin.
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