Poly(DL-Lactide-co-caprolactone) as drug carrier for antifungal agent Amphotericin B
Keywords:
Drug Delivery System, Poly(DL-lactide-co-caprolactone), Amphotericin B, MicrospheresAbstract
The objective of the present study is the preparation and characterization of Amphotericin B-loaded poly(DL-lactide-co-caprolactone) microspheres. The microspheres were prepared based on o/w emulsion solvent evaporation technique by varying the quantity of drug introduced. Characterization experiments included drug encapsulation efficiency, drug loading, particle size, morphology, FT-IR, stability, and drug release study. The drug encapsulation and drug loading increased with increasing drug mass. The microspheres exhibited homogeneous particle sizes with a spherical shape and porous surface. FT-IR spectrum of drug-loaded microspheres confirmed the absence of polymerdrug interaction. The stability study showed no major difference in the degradation of microspheres after 3 months of storage. Finally, the in vitro drug release behavior of the prepared formulations revealed a sustained release profile, with a burst effect for the formulations with the highest drug loadings.
References
. Kassab R, Yammine P, Moussa D.
New drug delivery system based on
Nystatin loaded poly(DL-lactide-cocaprolactone) microspheres. Asian J
Chem. 2011;23(7):3161-3164
. Kassab R, Yammine P, Moussa D.
Preparation and characterization of
antifungal drug-loaded poly(DLlactide-co-caprolactone) and poly(Llactide-co-caprolactone-coglycolide) microspheres. Int J Nov
Drug Deliv Tech. 2011;1(4):213-219
. Kassab R, Parrot-Lopez H, Fessi H,
Menaucourt J, Bonaly R, Coulon J.
Molecular recognition by
Kluyveromyces of Amphotericin Bloaded, galactose-tagged,
poly(Lactic Acid) microspheres.
Bioorg Med Chem.
;10(6):1767ă1775
. Paul M, Durand R, Fessi H, Rivollet
D, Houin R, Astier A, Deniau M.
Activity of a new liposomal
formulation of Amphotericin B
against two strains of Leishmania
infantum in a murine model.
Antimicrob Agents Chemother.
;41(8):1731-1734
. Alberto B, Kostas K, Charalambos
DP, Maurizio P. Biomdeical
applications for functionalized
nanotubes. Chem Commun.
;5:571-577.
. Alberto B, Kostas K, Maurizio P.
Applications of carbon nanotubes in
drug delivery. Expt Opin Drug Deliv.
;9(6):674-679
. Li M, Rouaud D, Poncelet D.
Microencapsulation by solvent
evaporation technique: state of the
art for process engineering
approaches. Int J Pharm.
;363(1-2):26-39
. Venkatesan P, Muralidaharan C,
Manavalan R, Valliappan K.
Selection of better method for the
preparation of microspheres by
applying analytic hierarchy process.
J Pharm Sci Res. 2009;1(3):64-78
. David WMM, Stephen JE, Eric JT.
Colchicine and antineoplastic
therapy for the prevention of
restenosis following percutaneous
coronary interventions. J Am Coll
Cardiol. 1991;17(6) Supplement
:126-131
. Huayu T, Zhaohui T, Xiuli Z, Xuesi
C, Xiabin J. Biodegradable synthetic
polymers: preparation,
functionalization and biomedical
application. Prog Polym Sci.
;37(2):237-280
. Nihant N, Grandfils C, Jerome R,
Teyssie P. Microencapsulation by
coacervation of PLGA. IV. Effect of
the processing parameters on
coacervation and encapsulation. J
Controlled
Release.1995;35(2,3):117-125
. Taizo F, Yoshitaka M, Tsunoru Y,
Yasuo S, Masaki O, Takashi N.
Biodegradation behavior of ultrahigh-strength hydroxyapatite/poly(Llactide) composite rods for internal
fixation of bone fractures.
Biomaterials.2000;21(9):889-898
. [13]. Isabel S, Carmen E, Matias L.
Preparation and evaluation of
insulin-loaded poly(DL-lactide)
microspheres using an experimental
design. Inter J Pharm.
;142(2):135-142
. Tadashia I, Youshiharu D.
Morphology and enzymatic
degradation of poly(L-lactic acid)
single crystals. Macromolecules.
;31(8):2461-2467
. Sinha VR, Bansal K, Kaushik R,
Kumria R, Trehan A. Poly(ē-
caprolactone) microspheres and
nanospheres: an overview. Inter J
Pharm. 2004;278(1):1-23
. Broz1 ME, VanderHart DL,
Washburn NR. Structure and
mechanical properties of poly(DLlactic acid)/poly(ē-caprolactone)
blends. Biomaterials.
;24(23):4181ă4190
. Hyo JL, Seung HC, Mun HN, Jeong
OL, In KL. Fabrication of an đ-lipoic
acid-eluting poly(DL-lactide-cocaprolactone) cuff for the inhibition
of neointimal formation. Exp Mol
Med 2009;41(1):25-32
. [18]. Das GS, Rao GHR, Wilson RF,
Chandy T. Colchicine encapsulation
with poly(ethyleneglycol)-coated
poly(lactic acid)/poly(ē-caprolactone)
microspheres- controlled release
studies. Drug Deliv. 2000;7(3):129-
. Chandy T, Wilson R, Rao GHR, Das
GS. Changes in Cisplatin delivery
due to surface-coated poly(lactic
acid)/poly(ē-caprolactone)
microspheres. J Biomater Appl.
;16(4):275-291
. Zhu KJ, Li Y, Jiang HL, Yasuda H,
Ichimaru A, Yamamoto K, Lecomte
P, Jerome R. Preparation,
characterization and in vitro release
properties of Ibuprofen-loaded
microspheres based on polylactide,
poly(ē-caprolactone) and their
copolymers. J Microencapsulation.
;22(1):25-36
. Ranne T, Tirri T, Yli-Urpo A, Narhi
TO. In vivo behavior of poly(ē-
caprolactone-co-DLlactide)/Bioactive Glass Composites
(BAG) in rat subcutaneous tissue. J
Bioact Compat Pol. 2007;22(3):249-
. Khatry S, Shastri N, Sadanandam
M. Novel drug delivery systems for
antifungal therapy. Int J Pharm
Pharm Sci. 2010;2(4):6-9
. Jay PJ, Neeraj K. Development of
Amphotericin B- loaded
polymersomes based on (PEG)3-
PLA copolymers: factors affecting
size and in vitro evaluation. Eur J
Pharm Sci. 2010;40(5):456-465
. Sunil A, Nadagouda M, Tejraj A.
Recent advances on chitosan-based
micro- and nanoparticles in drug
delivery. J Controlled Release.
;100(1):5-28
. Yoon Y, Kinam P. Control of
Encapsulation Efficiency and Initial
Burst in Polymeric Microparticle
Systems. Arch Pharmacal Res.
;27(1):1-12
