Biological properties of Pegylated PLA (PLA-PEG-PLA) and its capability for intracellular delivery of poor soluble peptide drug, gramicidin

Authors

  • Ebrahim Asadollahi Dept. of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
  • Asadolah Asadi Dept. of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
  • Farhood Najafi Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
  • Saber Zahri Dept. of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
  • Maryam Zohri Food Science and Technology Department, Science and Research Branch,Islamic Azad University ,Tehran ,IRAN
  • SeyedMehdi Hosseini Nasr Faculty of Pharmacy,Tehran University of Medical Science ,Tehran,Iran

Keywords:

PLA-PEG-PLA block co-polymer, hemolytic activity, biocompatibility, FITC, gramicidin, nanoparticle

Abstract

Poly lactic acid-co-poly ethylene glycol-co-Poly lactic acid (PLA-PEG-PLA) co-polymer was synthesized and after determination of its biological properties was used for intracellular delivery of poor soluble peptide drug, gramicidin. Toxicity of this polymer on LNCaP cell line was determined by MTT assay. Treatment of cells with fluorescein isothiocyanate (FITC) loaded nanoparticles and fluorescence microscopy indicates ability of this carrier for intracellular delivery. PLA-PEG-PLA nanoparticles containing poor soluble peptide drug, gramicidin, was prepared by solvent evaporation method. In order to confirm loading of gramicidin on PLA-PEG-PLA nanoparticles, FT-IR, spectrofluoremeteric, circular dichroism (CD), and scanning electron microscopy (SEM) studies were carried out. Our studies revealed treatment of prostate cancer cell line, LNCaP, with gramicidin loaded nanoparticles was more effective than gramicidin alone in killing cancer cells.

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Published

2012-06-30

How to Cite

Ebrahim Asadollahi, Asadolah Asadi, Farhood Najafi, Saber Zahri, Maryam Zohri, & SeyedMehdi Hosseini Nasr. (2012). Biological properties of Pegylated PLA (PLA-PEG-PLA) and its capability for intracellular delivery of poor soluble peptide drug, gramicidin. International Journal of Drug Delivery, 4(2), 257–265. Retrieved from https://ijdd.arjournals.org/index.php/ijdd/article/view/148

Issue

Vol. 4 No. 2 (2012): Apr-Jun

Section

Original Research Articles