Development of Zein-Pectin Nanoparticle as Drug carrier
Keywords:
Zein, Pectin, Nanoparticle, cytotoxicity, quercetin, ultrasonicationAbstract
Recent years have witnessed tremendous growth of nanotechnology based drug delivery system which reduces drug toxicity and side effects and increases the therapeutic index of the drug. Aim of the study is to develop a biodegradable, non-toxic nanoparticle, solely from natural polymers. Zein – pectin nanoparticle comprising of a hydrophobic zein core and a hydrophilic pectin shell was developed by ultrasonication method. SEM images confirm the nanosize of the nanoparticle. UVVisible and FT-IR spectroscopic results confirm the incorporation of zein, pectin and the encapsulation of the model drug quercetin in the nanoparticle. Zein is a prolamine class of protein found in wheat, maize etc and pectin is a polymer of galacturonic acid units found in plant cell wall
References
Kaparissides C, Alexandridou S, Kotti K and Chaitidou S. Recent advances in novel drug delivery systems. AZojono Journal of Nanotechnology online. 2006; 2: 1-11
De Jong WH and Borm JAP. Drug delivery and nanoparticles: Applications and hazards. International Journal of Nanomedicine. 2008; 3 (2): 133-149
Muller V, Piai JF, Fajarado AR, Favaro SL, Rubira AF and Muniz EC. Preparation and characterization of zein and zein - chitosan microspheres with great prospective of application in controlled drug release. Journal of Nanomaterials. 2011; 1-6
Jaya S, Durance TD and Wang R. Physical characterization of drug loaded microcapsules and controlled in vitro release study. The open biomaterials journal. 2010; 2: 9-17
Parris N, Cooke PH and Hicks KB. Encapsulation of essential oils in zein nanospherical particles. J. Agric. Food Chem. 2005; 53: 4788-4792
Dhanasingh S and Nallaperumal SK. Chitosan/casein microparticles: preparation, characterization and drug release studies. International Journal of Engineering and Applied Sciences. 2010; 6(4): 234 -238
Li P, Dai Ya-Ni, Zhang Jun-Ping, Wang Ai-Qin and Wei Qin. Chitosan- Alginate nanoparticles as a novel drug delivery system for nifedipine. Int J of Biomed Sci. 2008; 4(3): 221-228
Luo Y, Zhang B, Whent M, Yu L and Wang Q. Preparation and characterization of zein/chitosan complex for encapsulation of α – tocopherol, and its in vitro controlled release study. Colloids and Surfaces B: Biointerfaces. 2011; 85: 145 – 152
Shi L. and Gunasekaran S. Preparation of Pectin – ZnO nanocomposite. Nanoscale Res Lett. 2008; 3: 491 – 495
Reis AV, Guilherme MR, Paulino AT, Muniz EC, Mattoso LHC and Tambourgi EB. Synthesis of hollow – structured nano- and microspheres from pectin in a nanodroplet emulsion. Langmuir. 2009; 25: 2473 – 2478
Sudheesh S , Vijayalakshmi N R. Lipid lowering action of pectin from Cucumis sativus. Food Chemistry. 1999; 67: 281 -286
Presanna Kumar G, Sudheesh S, Vijayalakshmi NR. Hypoglycaemic effect of Coccinia indica: Mechanism of action. Planta Med. 1993; 59: 293 – 390
Mohamadzadeh J, Sadeghi-Mahoonak AR,Yaghbani M and Aalami M. Extraction of pectin from sunflower head residues of selected Iranian cultivars. World Applied Sciences Journal. 2010; 8(1): 21-24
Melton LD and Smith BG. Determining the degree of methylation and acetylation of pectin. Current Protocols in Food Analytical Chemistry. 2001; E3.4.1-E3.4.6
