Photoprotective herbal extract loaded nanovesicular creams inhibiting ultraviolet radiations induced photoaging
Keywords:
Herbal nanovesicles, ellagic acid, photo protective creams, photoaging, nanotransfersomesAbstract
Botanical photochemopreventives are used to fight against the adverse effects of ultra violet rays on skin which leads to photoaging. Phytoconstituents present in Punica granatum extract have antioxidant, anti-carcinogenic and photoprotective properties which was proved by estimation of phenolic content, sun protection factor and antioxidant effect determination of the alcoholic extract. Extract loaded nanotransfersomes were prepared, evaluated for size, entrapment efficiency and incorporated into the cream. The presence of vesicles in the cream was confirmed by the transmission electron microscopy and the efficacy of the formulated creams by the physicochemical parameters. The nanotransfersomal and conventional cream formulations were in vivo tested for change in skin hydration (Corneometer), viscoelasticity (Cutometer) and sebum content (Sebumeter) . The photoprotective effect of formulations was further confirmed by biochemical studies. Improved skin properties (viscoelasticity, skin hydration, sebum content) and biochemical parameters (superoxide dismutase, catalase, total protein and ascorbic acid level) with decreased malondialdehyde level was observed with nanotransfersomal cream formulations as compared to conventional creams. The efficacy was in the order nanotransfersomal creams > Conventional creams> Empty nanotransfersomal cream > Base cream.
References
Kligman LH, Kligman AM. The nature of photoaging: Its prevention and repair. Photodermatol. 1986; 3: 215-227.
Saraf S, Kaur CD. Phytoconstituents as photoprotective novel cosmetic formulations. Pharmacog Rev. 2010; 4, 7: 1-11.
Chanchal D, Saraf S. Herbal Photoprotective Formulations and their Evaluation. The Open Natural Products Journal 2009; 2: 71-76.
Ichihashi M. Photoaging of the skin, Anti-Aging Medicine 2009; 6 (6): 46-59.
Kaur CD, Saraf S. Development of photoprotective creams with antioxidant polyphenolic herbal extracts. Res J Med Plant. 2012; 6: 83-91.
Afaq F, Zaid M A, Khan N, Dreher M, Mukhtar H. Protective effect of pomegranate-derived products on UVB-mediated damage in human reconstituted skin. Exp Dermatol. 2009; 18: 553-561.
Park HM, Moon E, Kim AJ, Kim MH, Lee S, Lee JB et al. Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation. Int J Dermatol. 2010; 49: 276–282.
Festa F, Aglitti T, Duranti G, Ricordy R, Perticone P, Cozzi R. Strong antioxidant activity of ellagic acid in mammalian cells in vitro revealed by the comet assay. Anticancer Res. 2001; 21: 3903-3908.
Kaur IP, Kapila M, Agrawal R. Role of novel delivery systems in developing topical antioxidants as therapeutics to combat photoageing. Ageing Res Rev. 2007; 6: 271-288.
Chanchal D, Saraf S. Novel approaches in herbal cosmetics, J Cosmet Dermatol. 2008; 7: 89-95.
Betz G, Aeppli A, Menshutina N, Leuenberger H. In vivo comparison of various liposome formulations for cosmetic application. Int J Pharm. 2005; 296: 44-54.
Cevc G, Blume G. Lipid vesicles penetrate into intact skin owing to the transdermal osmotic gradients and hydration force. Biochim Biophys Acta. 1992; 1104: 226-232.
Ajazuddin, Saraf S. Applications of novel drug delivery system for herbal formulations. Fitoterapia 2010; 81: 680-689.
Rhie G, Shin MH, Seo JY, Choi WW, Cho KH, Kim KH, et al. Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo. J Invest Dermatol. 2001; 117: 1212-1217.
Harborne JB. Phytochemcial methods. Chapman and Hall Ltd., London, 1973, p. 49-188.
Kaur CD, Saraf S. Photochemoprotective activity of alcoholic extract of Camellia sinensis, Int J Pharmacol. 2011; 7, 3: 400-404.
Gao X, Ohlander M, Jeppsson N, Bjork L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem. 2000; 48: 1485-1490.
Elsayed MMA, Abdullah OY, Naggar VF, Khalafallah NM. Deformable liposome and ethosome: mechanism of enhanced skin delivery. Int J Pharm. 2006; 322: 60-66.
Forster T, Tesmann H. Phase inversion emulsification. Cosmet Toil. 1991; 11, 106.
Ashawat MS, Saraf S, Saraf S. Preparation and Characterisation of herbal creams for improvement of skin viscoelastic properties. Int J Cosmet Sci. 2008; 30: 183-193.
Kaur CD, Saraf S. Topical vesicular formulations of C. longa extract on recuperating the ultraviolet radiations damaged skin, J Cosmet Dermatol. 2011; 10: 260–265.
Dayan N, Touitou E. Carriers for skin delivery of trihexyphenidyl HCl: ethosomes vs. Liposomes. Biomaterials 2000; 21: 1879-1885
Hamad AWR, Momani WMA, Janakat S, Oran SA. Bioavailability of Ellagic Acid After Single Dose Administration Using HPLC. Pak J Nutrition. 2009; 8, 10: 1661-1664.
Kaur CD, Nahar M, Jain NK. Lymphatic targeting of zidovudine using surface engineered liposomes. J Drug Target. 2008; 16, 12: 798-805.
Indian Standard Bureau, 1999, IS: 11648, 5.2.
Kaur CD, Saraf S. In vitro SPF determination of herbal oils used in cosmetics. Pharmacog Res. 2010; 2, 1: 22-25.
Ashawat MS, Saraf S, Saraf S. Biochemical and histopathological studies of herbal cream against UV radiation induced damage. Trends Med Res. 2007; 2, 3:135-141.
Gupta NK, Dixit VK. Development and evaluation of vesicular system for curcumin delivery. Arch Dermatol Res. 2011; 303, 2: 89-101.
Sharma S, Kaur IP. Development and evaluation of sesamol as an antiaging agent. Int J Dermatol. 2006; 45: 200–208.
Agrawal R, Kaur IP. Inhibitory Effect of Encapsulated Curcumin on Ultraviolet-Induced Photoaging in Mice. Rejuv Res. 2010; 13, 3:1-14.
Winterboum CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med. 1975; 85: 337-341.
Ohkawa H, Onishi N, Yagi K. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351–358.
Natelson S. Techniques of Clinical Chemistry, Charlsc Thomas, US. 1971, p. 286.
Aebi H. Catalase. In: Bergmeyar (ed) Methods in enzymatic analysis. Academic Press, New York, 1974, p. 674-684.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949; 177: 751-766.
Kaur CD, Saraf S. Skin Care Assessment On The Basis Of Skin Hydration, Melanin, Erythema and Sebum at Various Body Sites, Int J Pharm Pharma Sci. 2011c; 3, 4 : 209-213.
Reiger M. The apparent pH of the skin. Cosmet Toilet. 1989; 104: 52-53.
Baird RM. Microbiological contamination of manufactured products: official and unofficial limits", in Microbial Quality assurance in Cosmetics, Toiletries and Non Sterile Pharmaceuticals, 2 nd ed., Baird R.M. and Bloomfield S.F. Eds. (Taylor and Francis, UK) 1996, p. 235-251.
Blichmann CW, Serup J, Winther A . Effects of single application of a moisturizer: evaporation of emulsion water, skin surface temperature, electrical conductance, electrical capacitance and skin surface (emulsion) lipids. Acta Derm Venereol. 1989; 69: 327-330.
Grove GL.The effects of moisturizers on skin surface hydration as measured in vivo by electrical conductivity. Curr Ther Res. 1991; 50: 5: 712-719.
Roding J, Ghyczy M. Control of skin humidity with liposomes. Stabilization of skin care oils and lipophilic active substances with liposomes. Seifen, O¨ le, Fette, Wachse 1991; 117: 10: 372–378.
Rawlings AV, Canestrari DA, Obkowski B. Moisturizer technology versus clinical performance. Dermatologic Therapy 2004; 17: 49–56.
Blank IH. Factors which influence the water content of the stratum corneum. J Invest Dermatol. 1952; 18:433-40.
Youn SW, Kim SJ, Hwang IA. Evaluation of facial skin type by sebum secretion: discrepancies between subjective descriptions and sebum secretion. Skin Res Technol. 2002; 8:168–172.
Sator PG, Schmidt JB, Honigsmann H. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J Am Acad Dermatol. 2003; 48:352-358.
